Saturation genome editing of BAP1 functionally classifies somatic and germline variants

Saturation genome editing of BAP1 functionally classifies somatic and germline variants

Optimized SGE approach improves experiment quality

We developed a HAP1 DNA ligase 4 (LIG4)-knockout (KO) line with genomic integration of a clonally derived Cas9 (HAP1-A5) and confirmed BAP1 essentiality in this line (Figs. 1 and 2a), high Cas9 activity (Fig. 2b and Extended Data Fig. 1a) and…

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