CRISPR-CasRx-mediated disruption of Aqp1/Adrb2/Rock1/Rock2 genes reduces intraocular pressure and retinal ganglion cell damage in mice

July 31, 2024 Health Trends, Health Trends - Australia

Plasmid construction

The CAG-CasRx-EGFP plasmid and U6-gRNA-mCherry cloning backbone were used. We synthesized gRNAs as single-stranded DNA oligos (all gRNA sequences are provided in Table S1). The gRNA oligos were annealed and cloned under the U6 promoter using the Eco31I enzyme (Thermo…

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CRISPR-CasRx-mediated disruption of Aqp1/Adrb2/Rock1/Rock2 genes reduces intraocular pressure and retinal ganglion cell damage in mice

Plasmid construction

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